What is Structured Illumination Microscopy? Structured Illumination Microscopy (SIM) is a super-resolution fluorescence microscopy technique that allows for imaging beyond the diffraction limit of ...

Top: Random Illumination Microscopy (RIM) utilizes random speckle patterns to achieve super-resolution imaging of biological tissues, improving resolution by a factor of two compared to the optical ...

SIMIP enables high-resolution images rich in both chemical and spatial information. A quantum cascade laser (QCL) excites molecular vibrations while a spatial light modulator (SLM) generates striped ...

A project at Swiss research center EPFL has designed an add-on unit intended to bring structured light capability to a standard optical microscope. Described in Nature Communications, the design ...

Nanoscopy is a field of microscopy that focuses on imaging and studying structures and processes at the nanoscale, typically below the diffraction limit of light. It encompasses various techniques ...

Even those who maintain that super-resolution microscopy is a powerful tool of biological discovery have admitted that it may have a bit of an image problem. For example, in a recent review, several ...

To unravel the complexities of biological phenomena, scientists have long relied on microscopy to visualize the intricate details of their specimens, including tissue architecture, cell morphology, ...

Fluorescence microscopy reveals cellular morphology and dynamics in remarkable detail, but achieving clear visualization at fast acquisition rates remains a challenge. A fundamental trade-off between ...

News Medical talks to Samuel Penwell and Winfried Wiegraebe about how the Vutara VXL enables robust, high-throughput 3D super-resolution via single-molecule localization, pairing bi-plane detection ...

SIM uses multiple images acquired under structured illumination to reconstruct a super-resolved image, exceeding the diffraction limit of traditional microscopy. However, SIM requires acquiring ...